Thin Layer Chromatography

In: Science

Submitted By ilioka
Words 2061
Pages 9
BAB I
PENDAHULUAN

1.1 LATAR BELAKANG
Perkembangan ilmu pengetahuan pada zaman sekarang telah berkembang dengan cukup pesat, terutama di bidang teknologi itu sendiri. Termasuk dalam bidang kedokteran itu sendiri. Pada makalah ini akan dibahas mengenai salah satu teknik ataupun metode yang digunakan dalam bidang kedokteran yaitu kromatografi terutama kromatografi lapis tipis.
Kromatografi adalah teknik kromatografi adalah teknik pemisahan suatu zat yang didasarkan pada perbadaan kecepatan migrasi komponen-komponen yang dipisahkan diantara dua fase, yaitu fase gerak dan fase diam. Istilah kromatografi pertama kali ditemukan oleh Michael Tswatt (1903), seorang ahli botani asal Rusia. Kromatografi sendiri berasal dari bahasa Yunani yang terdiri dari dua kata yaitu, “chromos” yang berarti warna dan “graphos” yang berarti menulis. Jadi kromatografi berarti penulisan dengan warna. Kromatografi sendiri dapat diklasifikasikan lagi menjadi empat teknik yaitu adalah Kromatografi Kertas (KKT), Kromatografi Lapis Tipis (KLT), Kromatografi Gas Cair (KGC), dan Kromatografi Cair Kinerja Tinggi (KCKT). Pada makalah ini akan dibahas mengenai kromatografi lapis tipis (KLT).
Kromatografi lapis tipis adalah salah satu teknik kromatografi yang banyak digunakan karena penggunaanya yang mudah dan cukup murah. Metode ini menggunakan lempeng kaca atau lembaran plastik yang ditutupi penyerap untuk lapisan tipis dan kering bentuk silika gel, alomina, selulosa dan polianida. Untuk menotolkan larutan cuplikan pada lempeng kaca, pada dasarnya dgunakan mikro pipet/ pipa kapiler. Setelah itu, bagian bawah dari lempeng dicelup dalam larutan pengulsi di dalam wadah yang tertutup (chamber) (Rudi, 2010)[1]

1.2 TUJUAN PENULISAN 1. Mengetahui apa itu kromatografi lapis tipis. 2. Mengetahui prinsip kerja dari kromatografi lapis tipis. 3. Mengetahui peran kromatografi…...

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