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In: English and Literature

Submitted By whytell
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Pages 5
Experiment 2: Centrifugation
Title: Centrifugation of fresh milk and Acetic Acid
Introduction:
Centrifugation is a process which materials suspended in a liquid medium undergo separation or concentrate due to the effect of gravity. Particles with different density and masses are settled at different rates in a tube which are response to gravity. Centrifuge is an instrument which can spin carrier vessels at high rotation speed and very high centrifugal force to allow centrifugation.
The centrifugal force generated is proportional to the rotation rate of the rotor and the distance between the rotor center and the centrifuge tube. There are three general classes of centrifuges: low speed, about 5000 rpm, high speed machine can up to about 25000 rpm and ultracentrifuges turn at up to
1000000 rpm. Microcentrifuge are common in laboratories which generate between 10000 rpm and 13000 rpm and 0.5 or 1.5 mL of disposable plastic tubes are used. Microcentrifuge can only setting for speed (rpm) but not relative centrifugal force (rcf): relative centrifugal force, g=(1.118× 10-5)rs2
(r=radius of the rotor in cm, s=speed of centrifuge in rpm)
Objective(s):
1.

To separate coagulated milk from mixture of fresh milk and Acetic Acid

by Centrifugation.
2.

To determine the relationship of speed of centrifugation with the

coagulation of milk which separate from mixture
3.

To study the process of centrifugation

Materials: Centrifuge, Falcon tube (50mL), Centrifuge tubes (15mL),
Microcentrifuge tubes (1.5mL), Micropipettes, blue tips (P-1000), Pipettes
(10mL & 5mL), Plastic Pipette Pump, Fresh Milk, 50% Acetic Acid
Procedure:
1.

10mL of 50% of acetic acid was drawn by pipettes with plastic pipette

pump and released into 50mL Falcon tube. 5mL of fresh milk was then drawn and released into falcon tube where 10mL of acetic acid contained.
2.…...

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